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Main sol. J593

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Internal Solution-ID: S4438

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.4 g 0.399 1.62
(NH4)2SO4 0.2 g 0.2 1.511
KCl 0.1 g 0.1 1.339
K2HPO4 0.1 g 0.1 0.573
FeSO4 x 7 H2O 25.0 g 24.95 89.744
Yeast extract 0.2 g 0.2 -
FeCl2 solution 1.0 ml - -
Trace element solution 1.0 ml - -
Distilled water 1000.0 ml - -
1 Adjust pH to 1.6 - 1.7 with 10 N H2SO4 and autoclave. After cooling, add filter-sterilized K2S4O6 solution to give a final concentration of 5 mM.
J593 MODIFIED 9K MEDIUM FOR FERROPLASMA AEOLICUM