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Main sol. J599

Internal Solution-ID: S4447

Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 10.000 g - -
Proteose peptone no. 3
(BD-Difco)
5.000 g - -
Glucose 1.000 g - -
NaCl 81.000 g - -
MgSO4 x 7 H2O 9.600 g - -
MgCl2 x 6 H2O 7.000 g - -
CaCl2 x 2 H2O 0.360 g - -
KCl 2.000 g - -
NaHCO3 0.060 g - -
NaBr 0.026 g - -
1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2. For preparation of solid medium, add 20.0 g/L agar.
J599 MODERATE HALOPHILE MEDIUM