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Artificial seawater

Internal Solution-ID: S4547

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 24.0 g 24 410.678
MgSO4 x 7 H2O 7.0 g 7 28.4
MgCl2 x 6 H2O 5.3 g 5.3 26.07
KCl 0.7 g 0.7 9.39
CaCl2 x 2 H2O 0.1 g 0.1 0.68
Distilled water 1000.0 ml - -
1 Adjust pH to 7.5.
2 Comment: For preparation of the liquid medium, use Marine broth 2216 (BD-Difco) instead of Marine agar 2216 and R2A broth instead of R2A agar by omitting agar from the R2A agar medium composition (see Medium No. 346).
J659 PELAGICOCCUS AGAR