Main sol. J665
Internal Solution-ID: S4553
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Poorly crystalline iron(III) oxide | 100.00 | mM | - | - | |
| NaHCO3 | 2.50 | g | 119.048 | 1417.12 | |
| NH4Cl | 0.25 | g | 11.905 | 222.561 | |
| NaH2PO4 x H2O | 0.60 | g | 28.571 | 238.141 | |
| KCl | 0.10 | g | 4.762 | 63.875 | |
|
Na2SeO4
(1 mM) |
1.00 | ml | - | - | |
| Vitamin solution | 10.00 | ml | - | - | |
| Trace mineral solution | 10.00 | ml | - | - | |
| 1 Add components to distilled water, bring volume to 1.0 L and adjust pH to 7.0. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, sparge with the same gas mixture for 10 - 15 min, seal with butyl rubber stoppers and autoclave. Before inoculation, add the following sterile solutions (autoclaved and stored under a N2 gas atmosphere) to the medium (per 10 ml of medium): | |||||
|
FeCl2
(130 mM) |
0.10 | ml | - | - | |
|
CaCl2 x 2 H2O
(0.4%) |
0.10 | ml | - | - | |
|
Sodium acetate
(pH 7.0, 2 M) |
0.10 | ml | - | - | |
Media with this solution
| J665 | FRESHWATER IRON GEL MEDIUM |