Main sol. J745
Internal Solution-ID: S4681
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 1000.00 | ml | - | - | |
| NaNO3 | 0.50 | g | 0.495 | 5.824 | |
| NH4Cl | 0.25 | g | 0.248 | 4.628 | |
|
Yeast extract
(BD-Difco) |
1.00 | g | 0.99 | - | |
|
Tryptone
(BD-Difco) |
1.00 | g | 0.99 | 13.929 | |
| Trace vitamins | 10.00 | ml | - | - | |
| 1 Mix components, adjust pH to 7.0 and autoclave. Replace the gas phase with N2-O2 (99:1, v/v) gas mixture, and pressurize to 200 kPa with the same gas mixture. | |||||
Media with this solution
| J745 | MJYP2 MEDIUM |