Main sol. J749
Internal Solution-ID: S4687
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.00 | g | 2.97 | 20.912 | |
| KH2PO4 | 0.20 | g | 0.198 | 1.455 | |
| NH4Cl | 0.30 | g | 0.297 | 5.553 | |
| NaCl | 21.00 | g | 20.792 | 355.785 | |
| MgCl2 x 6 H2O | 3.10 | g | 3.069 | 15.097 | |
| KCl | 0.50 | g | 0.495 | 6.641 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.149 | 1.01 | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 910.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
Sodium lactate
(1.0 M) |
20.00 | ml | - | - | |
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
|
NaHCO3
(*, 8%) |
60.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
| 2 Adjust pH to 7.1 - 7.4, if necessary. | |||||
Media with this solution
| J749 | DESULFOBULBUS MEDIUM WITH LACTATE |