Main sol. 215c
Internal Solution-ID: S472
Equipment needed: Gassing station
Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Brain heart infusion
(DIFCO) |
37.00 | g | 37 | - | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.423 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2. | |||||
Media with this solution
| 215c | BHI MEDIUM FOR STRICT ANAEROBES |