Main sol. J779
Internal Solution-ID: S4727
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 75.0 | g | 1071.43 | 18333.8 | |
| MgSO4 x 7 H2O | 8.8 | g | 125.714 | 510.047 | |
| KCl | 1.0 | g | 14.286 | 191.626 | |
| Yeast extract | 10.0 | g | 142.857 | - | |
|
Trypticase peptone
(BD-BBL) |
10.0 | g | 142.857 | - | |
| Trace vitamins | 10.0 | ml | - | - | |
| Trace minerals | 10.0 | ml | - | - | |
| 1 Add components to distilled water and bring volume to 1.0 L. Adjust pH 8.0 with NaOH. Autoclave under a N2 atmosphere. After cooling, add anaerobically the following solutions (autoclaved or *filter-sterilized): | |||||
|
Glucose
(10%) |
25.0 | ml | - | - | |
|
Thioglycolate-ascorbate solution
(*) |
25.0 | ml | - | - | |
| 2 Readjust pH to 8.0, if necessary. | |||||
Media with this solution
| J779 | MODIFIED HALOANAEROBIUM MEDIUM |