Basal minerals
Internal Solution-ID: S4878
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 190.0 | g | 3518.52 | 33197.3 | |
| NaHCO3 | 30.0 | g | 555.556 | 6613.21 | |
| NaCl | 16.0 | g | 296.296 | 5070.09 | |
| K2HPO4 | 2.0 | g | 37.037 | 212.641 | |
| 1 Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Then, aseptically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
Trace vitamins (see Medium No. 197)
(*) |
10.0 | ml | - | - | |
|
NH4Cl
(4 M) |
1.0 | ml | - | - | |
|
Selenite-tungstate solution (see Medium No. 431)
(*) |
1.0 | ml | - | - | |
| Trace element solution SL-4 | 1.0 | ml | - | - | |
|
MgSO4
(1 M) |
1.0 | ml | - | - | |
|
Yeast extract
(10%) |
0.2 | ml | - | - | |
|
Sodium butyrate
(1 M) |
20.0 | ml | - | - | |
|
Sodium acetate
(1 M) |
20.0 | ml | - | - | |
| 2 Distribute the medium into culture vessels, replace the gas phase with N2 containing 5% O2 (by vol.) and close with butyl rubber stoppers. | |||||
Media with this solution
| J887 | NATRONOLIMNOBIUS AHT32 MEDIUM (A) |