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Main sol. 230

Internal Solution-ID: S490

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tryptone 10.0 g 10 140.687
Yeast extract 10.0 g 10 -
K2HPO4 0.5 g 0.5 2.871
KH2PO4 0.5 g 0.5 3.674
NaCl 1.0 g 1 17.112
MgSO4 0.1 g 0.1 0.831
CaCl2 0.1 g 0.1 0.901
NaHCO3 6.0 g 6 71.423
Resazurin 0.1 mg 1.0e-4 4.4e-4
L-Cysteine HCl x H2O 0.3 g 0.3 1.708
Arginine 3.0 g 3 17.222
Distilled water 1000.0 ml - -
1 Adjust pH to 7.2.
230 TRYPTONE YEAST EXTRACT MINERAL MEDIUM