Main sol. J906
Internal Solution-ID: S4907
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trisodium citrate x 2 H2O | 1.00 | g | 9.901 | - | |
|
Tryptone
(BD-Difco) |
2.00 | g | 19.802 | 278.587 | |
|
Peptone
(Oxoid) |
1.00 | g | 9.901 | - | |
| KH2PO4 | 0.60 | g | 5.941 | 43.653 | |
| NH4Cl | 0.60 | g | 5.941 | 111.06 | |
| NaCl | 200.00 | g | 1980.2 | 33884.3 | |
| MgCl2 x 6 H2O | 0.20 | g | 1.98 | 9.74 | |
| K2SO4 | 20.00 | g | 198.02 | 1136.35 | |
| FeCl2 x 4 H2O | 36.00 | mg | 0.356 | 1.793 | |
| MnCl2 x 4 H2O | 0.36 | mg | 0.004 | - | |
| SL-6 trace element solution | 1.00 | ml | - | - | |
| 1 Add components to distilled water and bring volume to 900 ml. Adjust pH to 7.0 and autoclave. For preparation of solid medium, add 20.0 g/L Bacto agar (BD-Difco). Separately autoclave the following solution: | |||||
|
Glycine-NaOH buffer
(pH 10.0, 0.5 M) |
100.00 | ml | - | - | |
| 2 After autoclaving, add the solution to the medium. | |||||
Media with this solution
| J906 | MK MEDIUM |