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Main sol. J988

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Internal Solution-ID: S5018

Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 0.53 g 0.524 3.689
KH2PO4 0.41 g 0.405 2.977
NH4Cl 0.30 g 0.296 5.542
CaCl2 x 2 H2O 0.11 g 0.109 0.739
MgCl2 x 6 H2O 0.10 g 0.099 0.486
NaCl 0.30 g 0.296 5.073
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract
(BD-Difco) 		0.40 g 0.395 -
Resazurin 0.50 mg 4.9e-4 0.002
NaHCO3 4.00 g 3.953 47.051
Trace vitamins 10.00 ml - -
Glycerol 2.30 g 2.273 24.679
Na2S x 9 H2O 0.50 g 0.494 2.057
Distilled water 1000.00 ml - -
1 Mix ingredients except Trace vitamins, NaHCO3 and Na2S x 9H2O. Separately autoclave NaHCO3 and Na2S x 9H2O (4 and 5% solutions, respectively) under a N2-CO2 atmosphere. Anaerobically and aseptically add the NaHCO3 and Na2S x 9H2O solutions, and filter-sterilized Trace vitamins to the medium. Check final pH to be 7.0 - 7.2. Pressurize the inoculated bottles to 70 kPa N2-CO2 (4:1, v/v).
J988 MPOB MEDIUM