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Main sol. J1002

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Internal Solution-ID: S5038

Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco) 		5.00 g 4.97 -
Tryptone
(BD-Difco) 		5.00 g 4.97 69.924
Sodium pyruvate 3.00 g 2.982 27.1
NaCl 19.00 g 18.887 323.181
MgCl2 x 6 H2O 9.00 g 8.946 44.005
MgSO4 x 7 H2O 0.15 g 0.149 0.605
CaCl2 x 2 H2O 0.30 g 0.298 2.028
KCl 0.50 g 0.497 6.667
KH2PO4 0.42 g 0.417 3.068
(NH4)2SO4 0.10 g 0.099 0.752
NaBr 0.05 g 0.05 0.483
SrCl2 x 6 H2O 0.02 g 0.02 0.075
Na2SeO4 0.02 g 0.02 0.105
Wolfe's modified mineral elixir 5.00 ml - -
Vitamin solution 0.50 ml - -
PIPES 6.05 g 6.014 19.887
L-Cysteine HCl x H2O 0.50 g 0.497 -
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 1000.00 ml - -
1 Adjust pH to 7.3.
2 Mix ingredients, except L-cysteine x HCl x H2O, and bring to the boil while gassing with N2-H2 (95:5) gas mixture. When the color turns from blue to reddish pink, add L-cysteine x HCl x H2O during continuous gassing and boiling. Anaerobically dispense the medium in tubes under a N2-H2 (95:5) atmosphere. Stopper the tubes and autoclave.
J1002 YTPS MEDIUM