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Main sol. J1012

Internal Solution-ID: S5054

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
UBS solution 100.0 ml - -
Trace minerals 10.0 ml - -
Ni-Se-W solution 10.0 ml - -
Glucose 0.9 g 0.8 4.441
Yeast extract 0.2 g 0.178 -
Distilled water 1000.0 ml - -
1 Mix components thoroughly, adjust pH 2.5 with H2SO4 and autoclave. After cooling, add the following solution (filter-sterilized) to the medium:
FeSO4
(pH 2.0, 1 M)
5.0 ml - -
J1012 ACIDITHRIX FERROOYDANS MEDIUM