Main sol. J1013
Internal Solution-ID: S5055
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Basal medium | 950.00 | ml | - | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Vitamin solution
(see Medium No. 197) |
10.00 | ml | - | - | |
|
NaHCO3
(5%, w/v) |
30.00 | ml | - | - | |
|
Sucrose
(20%, w/v) |
10.00 | ml | - | - | |
| Yeast extract | 50.00 | mg | 0.05 | - | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.039 | |
| 1 Autoclave Basal medium supplemented with Trace element solution SL-10 and Selenite-tungstate solution under 100% N2 gas. Separately autoclave sucrose and yeast extract, and add to the medium. Filter-sterilize Vitamin solution and NaHCO3 solution, and add to the medium. Distribute the medium into culture vessels under 100% N2 gas and seal with butyl rubber stoppers. Before inoculation, reduce the medium with 5% Na2S x 9H2O solution (autoclaved under N2 gas). Readjust pH to 7.7 - 7.9, if necessary. | |||||
Media with this solution
| J1013 | ACETIVIBRIO MEDIUM |