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JCM 1013 . ACETIVIBRIO MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1013 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basal medium 950.00 ml - -
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Vitamin solution
(see Medium No. 197) 		10.00 ml - -
NaHCO3
(5%, w/v) 		30.00 ml - -
Sucrose
(20%, w/v) 		10.00 ml - -
Yeast extract 50.00 mg 0.05 -
Na2S x 9 H2O 0.25 g 0.25 1.039
1 Autoclave Basal medium supplemented with Trace element solution SL-10 and Selenite-tungstate solution under 100% N2 gas. Separately autoclave sucrose and yeast extract, and add to the medium. Filter-sterilize Vitamin solution and NaHCO3 solution, and add to the medium. Distribute the medium into culture vessels under 100% N2 gas and seal with butyl rubber stoppers. Before inoculation, reduce the medium with 5% Na2S x 9H2O solution (autoclaved under N2 gas). Readjust pH to 7.7 - 7.9, if necessary.

Basal medium #

950
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.40 g 1.474 10.829
NH4Cl 0.50 g 0.526 9.84
MgCl2 x 6 H2O 0.20 g 0.211 1.036
CaCl2 x 2 H2O 0.15 g 0.158 1.074
Resazurin 1.00 mg 0.001 0.005
Distilled water 950.00 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 20 other media (first defined in Medium J433).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8
Equipment needed: Autoclave, Filter
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 1.397 10.267
NH4Cl 0.499 9.329
Na2S x 9 H2O 0.25 1.039
MgCl2 x 6 H2O 0.2 0.982
CaCl2 x 2 H2O 0.15 1.018
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain