Name: PSEUDOMONAS HALOPHILA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J433 #

Solution A #

901

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	46.8	g	51.942	888.814
MgSO₄ x 7 H₂O	39.4	g	43.729	177.418
NH₄Cl	1.0	g	1.11	20.749
Glycerol	5.0	g	5.549	60.261
Trace element solution SL-10	1.0	ml	-	-
Vitamin solution (filter-sterilized)	10.0	ml	-	-
Distilled water	890.0	ml	-	-

100

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	1	g	10	73.483
Distilled water	100	ml	-	-

1000

Solution occurs in 20 other media (first defined in Medium J433).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%, 7.7 M)	10.0	ml	2.5	68.568
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
ZnCl₂	70.0	mg	0.07	0.514
MnCl₂ x 4 H₂O	100.0	mg	0.1	0.505
H₃BO₃	6.0	mg	0.006	0.097
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	990.0	ml	-	-
1 Dissolve FeCl₂ x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

1000

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Biotin	2.00	mg	0.002	0.008
Folic acid	2.00	mg	0.002	0.005
Pyridoxine hydrochloride	10.00	mg	0.01	-
Thiamine-HCl x 2 H₂O	5.00	mg	0.005	-
Riboflavin	5.00	mg	0.005	0.013
Nicotinic acid	5.00	mg	0.005	0.041
Calcium pantothenate	5.00	mg	0.005	0.01
p-Aminobenzoic acid	1.00	mg	1.0e-3	0.007
Vitamin B₁₂	0.01	mg	1.0e-5	7.4e-6
Distilled water	1000.00	ml	-	-
1 Autoclave Solutions A and B separately and combine after cooling. Filter-sterilize vitamin solution and add to the autoclaved medium.
2 Adjust pH to 7.0.