Vitamin solution
Internal Solution-ID: S4179
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Biotin | 2.00 | mg | 0.002 | 0.008 | |
| Folic acid | 2.00 | mg | 0.002 | 0.005 | |
| Pyridoxine hydrochloride | 10.00 | mg | 0.01 | - | |
| Thiamine-HCl x 2 H2O | 5.00 | mg | 0.005 | - | |
| Riboflavin | 5.00 | mg | 0.005 | 0.013 | |
| Nicotinic acid | 5.00 | mg | 0.005 | 0.041 | |
| Calcium pantothenate | 5.00 | mg | 0.005 | 0.01 | |
| p-Aminobenzoic acid | 1.00 | mg | 1.0e-3 | 0.007 | |
| Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Autoclave Solutions A and B separately and combine after cooling. Filter-sterilize vitamin solution and add to the autoclaved medium. | |||||
| 2 Adjust pH to 7.0. | |||||
Media with this solution
| J433 | PSEUDOMONAS HALOPHILA MEDIUM |