Main sol. 246
Internal Solution-ID: S507
This solution is used in 2 media and was first defined in medium 246.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Beef extract | 10 | g | 10 | - | |
| Peptone | 10 | g | 10 | - | |
| Agar | 20 | g | 20 | 59.465 | |
| Tap water | 250 | ml | - | - | |
| Sea water | 750 | ml | - | - | |
| 1 Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Directly after autoclaving, add warm (55°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. | |||||
| 2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. | |||||