This medium was extracted automatically and has not been curated by experts, yet.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
|1 Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Directly after autoclaving, add warm (55°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature.|
|2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.|
|Medium type:||Complex medium|
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|Compound||Conc. [g/L]||Conc. [mM]|