DSMZ 243 . SEAWATER YEAST PEPTONE AGAR

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Yeast extract 3 g 3 -
Agar 12 g 12 35.679
Distilled water 250 ml - -
Sea water
(filtered, aged)
750 ml - -
1 Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3.
2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.
Solution occurs in 5 other media (first defined in Medium 243).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 28.13 g 28.13 481.348
KCl 0.77 g 0.77 10.329
CaCl2 x 2 H2O 1.60 g 1.6 10.883
MgCl2 x 6 H2O 4.80 g 4.8 23.61
NaHCO3 0.11 g 0.11 1.309
MgSO4 x 7 H2O 3.50 g 3.5 14.2
Distilled water 1000.00 ml - -

Metadata

Last modified: 08.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
Peptone 5 -
Yeast extract 3 -
Distilled water - -
Sea water - -

Cultivation metadata from 5 strains BacDive

phyla 2 5 strains
temperature mesophilic 5 strains
temperature psychrophilic 1 strain