Main sol. 243
Internal Solution-ID: S504
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Yeast extract | 3 | g | 3 | - | |
| Agar | 12 | g | 12 | 35.679 | |
| Distilled water | 250 | ml | - | - | |
|
Sea water
(filtered, aged) |
750 | ml | - | - | |
| 1 Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3. | |||||
| 2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. | |||||
Media with this solution
| 243 | SEAWATER YEAST PEPTONE AGAR |