246a
246a .
REACTIVATION WITH LIQUID MEDIUM 246
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 246a #
ml
Equipment needed: Autoclave
| 1 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 246. Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Beef extract | 10 | g | 10 | - | |
| Peptone | 10 | g | 10 | - | |
| Agar | 20 | g | 20 | 59.465 | |
| Tap water | 250 | ml | - | - | |
| Sea water | 750 | ml | - | - | |
| 2 Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. | |||||
| 3 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. | |||||
Solution occurs in 5
other media (first defined in Medium 243).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 28.13 | g | 28.13 | 481.348 |
| KCl | 0.77 | g | 0.77 | 10.329 |
| CaCl2 x 2 H2O | 1.60 | g | 1.6 | 10.883 |
| MgCl2 x 6 H2O | 4.80 | g | 4.8 | 23.61 |
| NaHCO3 | 0.11 | g | 0.11 | 1.309 |
| MgSO4 x 7 H2O | 3.50 | g | 3.5 | 14.2 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 08.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | 59.465 |
| Beef extract | 10 | - |
| Peptone | 10 | - |
| Tap water | - | - |
| Sea water | - | - |
