DSMZ 246a . REACTIVATION WITH LIQUID MEDIUM 246

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
1 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 246. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Beef extract 10 g 10 -
Peptone 10 g 10 -
Agar 20 g 20 59.465
Tap water 250 ml - -
Sea water 750 ml - -
2 Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature.
3 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.
Solution occurs in 5 other media (first defined in Medium 243).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 28.13 g 28.13 481.348
KCl 0.77 g 0.77 10.329
CaCl2 x 2 H2O 1.60 g 1.6 10.883
MgCl2 x 6 H2O 4.80 g 4.8 23.61
NaHCO3 0.11 g 0.11 1.309
MgSO4 x 7 H2O 3.50 g 3.5 14.2
Distilled water 1000.00 ml - -

Metadata

Last modified: 08.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.465
Beef extract 10 -
Peptone 10 -
Tap water - -
Sea water - -

Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen aerobe 1 strain
temperature psychrophilic 3 strains
temperature mesophilic 1 strain