Main sol. 246a
Internal Solution-ID: S510
Equipment needed: Autoclave
| 1 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 246. Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Beef extract | 10 | g | 10 | - | |
| Peptone | 10 | g | 10 | - | |
| Agar | 20 | g | 20 | 59.465 | |
| Tap water | 250 | ml | - | - | |
| Sea water | 750 | ml | - | - | |
| 2 Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. | |||||
| 3 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. | |||||
Media with this solution
| 246a | REACTIVATION WITH LIQUID MEDIUM 246 |