Main sol. J1031
Internal Solution-ID: S5084
Equipment needed: Autoclave
Anaerobic workbench
| 1 Bubble N2 for 3 - 4 min and check pH to be 7.6 - 8.0. Readjust pH to the range if necessary. Autoclave under a N2 gas atmosphere. Aseptically and anaerobically add the following solution to the medium (separately autoclaved under N2): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.330 | g | 0.311 | 5.82 | |
| KCl | 0.330 | g | 0.311 | 4.176 | |
| MgCl2 x 6 H2O | 0.330 | g | 0.311 | 1.531 | |
| KH2PO4 | 0.165 | g | 0.156 | 1.144 | |
| CaCl2 x 2 H2O | 0.033 | g | 0.031 | 0.212 | |
| Trace elements solution | 10.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 2 Mix components, bring to a boil and cool down under a N2 (100%) gas stream. While gassing, add the following components to the medium: | |||||
| Trace vitamins | 10.000 | ml | - | - | |
| NaHCO3 | 2.000 | g | 1.887 | 22.46 | |
|
Glucose
(w/v, 10%) |
20.000 | ml | - | - | |
|
Yeast extract
(1%) |
10.000 | ml | - | - | |
|
KNO3
(1 M) |
10.000 | ml | - | - | |
Media with this solution
| J1031 | THERMOGUTTA HYPOGEA MEDIUM |