Main sol. J1032
Internal Solution-ID: S5085
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 18.000 | g | 17.143 | 293.341 | |
| MgCl2 x 6 H2O | 4.330 | g | 4.124 | 20.284 | |
| NH4Cl | 0.330 | g | 0.314 | 5.876 | |
| KCl | 0.330 | g | 0.314 | 4.216 | |
| KH2PO4 | 0.165 | g | 0.157 | 1.155 | |
| CaCl2 x 2 H2O | 0.033 | g | 0.031 | 0.214 | |
| Trace elements solution | 10.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components, bring to a boil and cool down under a CO2 (100%) gas stream. While gassing, add the following components to the medium: | |||||
| Trace vitamins | 10.000 | ml | - | - | |
| NaHCO3 | 2.000 | g | 1.905 | 22.674 | |
| 2 Bubble N2 for 3 - 4 min and check pH to be 6.5 - 6.8. Readjust pH to the range if necessary. Autoclave under a CO2 gas atmosphere. Add the following solution to the medium (separately autoclaved under N2): | |||||
|
Maltose
(w/v, 10%) |
20.000 | ml | - | - | |
|
KNO3
(1 M) |
10.000 | ml | - | - | |
Media with this solution
| J1032 | SVX8 MEDIUM |