Main sol. J1047
Internal Solution-ID: S5108
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(Sigma) |
30.0 | g | 29.014 | - | |
| PIPES | 6.0 | g | 5.803 | 19.189 | |
| Yeast extract | 1.0 | g | 0.967 | - | |
|
Tryptone
(BD-Difco) |
1.0 | g | 0.967 | 13.606 | |
| Resazurin | 0.5 | mg | 4.8e-4 | 0.002 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components and adjust pH to 7.0. Bubble the medium with N2 for 5 min, then distribute the medium into culture vessels under N2, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions aseptically and anaerobically (autoclaved and stored under a N2 gas atmosphere): | |||||
|
Glucose
(1.0 M) |
14.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
Media with this solution
| J1047 | MARINITOGA MEDIUM |