Main sol. J1063
Internal Solution-ID: S5128
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(Sigma) |
30.00 | g | 30 | - | |
| KH2PO4 | 0.15 | g | 0.15 | 1.102 | |
| K2HPO4 | 0.15 | g | 0.15 | 0.861 | |
| NH4Cl | 2.00 | g | 2 | 37.39 | |
| Yeast extract | 2.00 | g | 2 | - | |
| Glucose | 5.00 | g | 5 | 27.753 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
| NaHCO3 | 1.00 | g | 1 | 11.904 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components except NaHCO3 and adjust pH to 7.0. Bring to a boil, cool down to room temperature under a N2-CO2 (4:1, v/v) gas stream and add NaHCO3. Dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
Media with this solution
| J1063 | SEA SALTS YEAST EXTRACT GLUCOSE MEDIUM |