Main sol. J1076
Internal Solution-ID: S5141
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 20.0 | g | 19.861 | 339.852 | |
NH4Cl | 1.0 | g | 0.993 | 18.565 | |
KH2PO4 | 0.1 | g | 0.099 | 0.73 | |
K2HPO4 | 0.1 | g | 0.099 | 0.57 | |
KCl | 0.1 | g | 0.099 | 1.332 | |
MgCl2 x 6 H2O | 0.1 | g | 0.099 | 0.488 | |
Tris base | 3.0 | g | 2.979 | 24.593 | |
Na2S2O3 x 5 H2O | 3.0 | g | 2.979 | 12.004 | |
Trace element solution | 1.0 | ml | - | - | |
Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
Distilled water | 950.0 | ml | - | - | |
1 Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N2 atmosphere): | |||||
Yeast extract
(w/v, 10%) |
10.0 | ml | - | - | |
Maltose
(0.5 M) |
20.0 | ml | - | - | |
Na2CO3
(8%) |
20.0 | ml | - | - | |
Na2S x 9 H2O
(5%) |
6.0 | ml | - | - | |
2 Check final pH be around 8.2. |
J1076 | ALKALIPHILUS LACT MEDIUM |