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Main sol. J1076

Internal Solution-ID: S5141

Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.0 g 19.861 339.852
NH4Cl 1.0 g 0.993 18.565
KH2PO4 0.1 g 0.099 0.73
K2HPO4 0.1 g 0.099 0.57
KCl 0.1 g 0.099 1.332
MgCl2 x 6 H2O 0.1 g 0.099 0.488
Tris base 3.0 g 2.979 24.593
Na2S2O3 x 5 H2O 3.0 g 2.979 12.004
Trace element solution 1.0 ml - -
Resazurin 1.0 mg 9.9e-4 0.004
Distilled water 950.0 ml - -
1 Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N2 atmosphere):
Yeast extract
(w/v, 10%)
10.0 ml - -
Maltose
(0.5 M)
20.0 ml - -
Na2CO3
(8%)
20.0 ml - -
Na2S x 9 H2O
(5%)
6.0 ml - -
2 Check final pH be around 8.2.
J1076 ALKALIPHILUS LACT MEDIUM