Artificial seawater
Internal Solution-ID: S5211
This solution is used in 3 media and was first defined in medium J1118.
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Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.30 | g | 26.3 | 450.034 | |
| CaCl2 x 2 H2O | 1.54 | g | 1.54 | 10.475 | |
| KBr | 0.10 | g | 0.1 | 0.84 | |
| KF | 4.00 | mg | 0.004 | 0.014 | |
| KCl | 0.70 | g | 0.7 | 9.39 | |
| H3BO3 | 30.00 | mg | 0.03 | 0.485 | |
| MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
| KHCO3 | 0.24 | g | 0.24 | 2.397 | |
| SrCl2 x 6 H2O | 17.00 | mg | 0.017 | 0.064 | |
| MgCl2 x 6 H2O | 5.20 | g | 5.2 | 25.578 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Artificial seawater can be replaced with natural seawater. In this case, filtrate seawater (pore size, 02 �m) and boil. After cooling, filtrate again (pore size, 0.45 �m). | |||||