Main sol. J1155
Internal Solution-ID: S5265
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.495 | 3.638 | |
| K2HPO4 | 0.50 | g | 0.495 | 2.842 | |
| NH4Cl | 0.50 | g | 0.495 | 9.255 | |
| NaCl | 5.00 | g | 4.951 | 84.711 | |
| Na2SO4 | 2.84 | g | 2.812 | 19.796 | |
| MgCl2 x 6 H2O | 0.30 | g | 0.297 | 1.461 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.337 | |
| KCl | 0.40 | g | 0.396 | 5.312 | |
|
Trace element solution
(see Medium No. 294) |
1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 955.00 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.00 | ml | - | - | |
|
Sodium propionate
(1 M) |
20.00 | ml | - | - | |
|
Trace vitamins
(*) |
1.00 | ml | - | - | |
| 2 Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N2): | |||||
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
| 3 Readjust pH to 7.6, if necessary. | |||||
Media with this solution
| J1155 | DESULFOOBULBUS OLIGOTROPHICUS MEDIUM |