Main sol. J1180
Internal Solution-ID: S5291
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 128.00 | g | 4571.43 | 78224.3 | |
| Na2CO3 | 95.00 | g | 3392.86 | 32011.7 | |
| NaHCO3 | 15.00 | g | 535.714 | 6377.02 | |
| KCl | 2.50 | g | 89.286 | 1197.66 | |
| K2HPO4 | 1.75 | g | 62.5 | 358.832 | |
| 1 Add components to distilled water and bring volume to 1.0 L. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
|
NH4Cl
(10%) |
1.25 | ml | - | - | |
|
MgSO4
(1 M) |
1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
|
Trace vitamins
(*) |
5.00 | ml | - | - | |
|
Yeast extract
(10%) |
0.20 | ml | - | - | |
|
Cellobiose
(10%) |
20.00 | ml | - | - | |
Media with this solution
| J1180 | CELLULOLYTIC NATRONOARCHAEA MEDIUM |