Main sol. J1192
Internal Solution-ID: S5305
Equipment needed: Filter
Autoclave
Anaerobic workbench
| 1 Mix components thoroughly, distribute the medium in a culture bottle under a H2-CO2 (4:1, v/v) gas stream, seal with a butyl rubber stopper and autoclave. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 15.00 | g | 14.955 | 255.906 | |
| MgCl2 x 6 H2O | 4.00 | g | 3.988 | 19.616 | |
| KCl | 0.33 | g | 0.329 | 4.413 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.329 | 2.238 | |
| (NH4)2SO4 | 0.50 | g | 0.499 | 3.773 | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Distilled water | 840.00 | ml | - | - | |
| Iron(III) citrate solution | 100.00 | ml | - | - | |
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
|
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
|
KH2PO4
(0.1 M) |
20.00 | ml | - | - | |
Media with this solution
| J1192 | GEOTHERMOBACTER MEDIUM |