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Main sol. 266

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Internal Solution-ID: S532

Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.3 9.838
KH2PO4 0.28 g 0.28 2.058
MgSO4 x 7 H2O 0.25 g 0.25 1.014
CaCl2 x 2 H2O 0.07 g 0.07 0.476
FeCl3 x 6 H2O 0.02 g 0.02 0.074
MnCl2 x 4 H2O 1.80 mg 0.002 0.009
Na2B4O7 x 10 H2O 4.50 mg 0.005 -
ZnSO4 x 7 H2O 0.22 mg 2.2e-4 7.7e-4
CuCl2 x 2 H2O 0.05 mg 5.0e-5 2.9e-4
Na2MoO4 x 2 H2O 0.03 mg 3.0e-5 1.2e-4
VOSO4 x 2 H2O 0.03 mg 3.0e-5 1.5e-4
CoSO4 x 7 H2O 0.01 mg 1.0e-5 6.5e-5
NaCl 40.00 g 40 684.463
Resazurin 1.00 mg 1.0e-3 0.004
Yeast extract 2.00 g 2 -
Sulfur
(powdered) 		5.00 g 5 155.909
Distilled water 1000.00 ml - -
1 Adjust final pH to 5.8.
2 Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for a few minutes not autoclaved; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere.
266 THERMOCOCCUS CELER MEDIUM