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Main sol. J1239

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Internal Solution-ID: S5374

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 3.00 g 3 22.045
MgSO4 x 7 H2O 1.00 g 1 4.057
CaCl2 x 2 H2O 0.25 g 0.25 1.701
(NH4)2SO4 0.20 g 0.2 1.514
Yeast extract 0.10 g 0.1 -
Sucrose 5.00 g 5 14.607
Sulfur
(powder) 		4.00 g 4 124.727
Distilled water 1000.00 ml - -
1 Mix components except sulfur, yeast extract and sucrose, adjust pH to 2.5 with 10 N H2SO4 and autoclave. Separately autoclave yeast extract and sucrose and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) containing appropriate amount of sulfur, replace gas phase with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers.
J1239 THERMOPLASMA KAMCHATKENSIS MEDIUM