Main sol. J1249
Internal Solution-ID: S5385
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 10.00 | g | 9.99 | 140.546 | |
| Beef extract | 10.00 | g | 9.99 | - | |
| Yeast extract | 5.00 | g | 4.995 | - | |
| Malt extract | 150.00 | g | 149.85 | - | |
| Glucose | 20.00 | g | 19.98 | 110.902 | |
| Diammonium hydrogen citrate | 2.00 | g | 1.998 | 8.834 | |
| Sodium acetate | 5.00 | g | 4.995 | 60.892 | |
| K2HPO4 | 2.00 | g | 1.998 | 11.471 | |
| MnSO4 x n H2O | 0.19 | g | 0.19 | - | |
| MgSO4 x 7 H2O | 0.58 | g | 0.579 | 2.351 | |
| Tween 80 | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 1.00 | g | 0.999 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 After autoclaving, aseptically add 20 ml acetic acid (filter-sterilized). | |||||
Media with this solution
| J1249 | TYG-ACETATE MEDIUM |