Main sol. J1258
Internal Solution-ID: S5397
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 4.00 | g | 3.802 | 26.769 | |
| KH2PO4 | 0.20 | g | 0.19 | 1.397 | |
| NH4Cl | 0.25 | g | 0.238 | 4.443 | |
| NaCl | 20.00 | g | 19.011 | 325.315 | |
| MgCl2 x 6 H2O | 3.40 | g | 3.232 | 15.897 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.238 | 1.616 | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Resazurin | 0.50 | mg | 4.8e-4 | 0.002 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 gas atmosphere; **filter-sterilized and stored under an 80% N2-20% CO2 gas mixture): | |||||
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
|
Vitamin B12 solution 0.05 g/L
(*) |
1.00 | ml | - | - | |
|
Steric solution
(see below) |
10.00 | ml | - | - | |
|
NaHCO3
(**, 8%) |
20.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Media with this solution
| J1258 | DESULFOBACTERIUM OLEOVORANS MEDIUM |