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Stearic solution

Internal Solution-ID: S5398

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Stearic acid 0.36 g 32.727 115.034
NaOH
(2 N)
0.63 ml - -
Distilled water 10.00 ml - -
1 Heat the solution in a closed vial under a N2 atmosphere in a boiling water bath. Shake until stearic acid is dissolved and autoclave. Add the solution to the medium while stearic acid is dissolved. Re-melt if stearic acid is solidified.
J1258 DESULFOBACTERIUM OLEOVORANS MEDIUM