Main sol. J1287
Internal Solution-ID: S5434
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Base salt medium | 750.0 | ml | - | - | |
| Base soda medium | 250.0 | ml | - | - | |
| 1 Mix two media autoclaved separately. Aseptically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
MgSO4
(1 M) |
1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
|
Selenite-tungstate solution
(*) |
1.0 | ml | - | - | |
|
Trace vitamins
(*) |
5.0 | ml | - | - | |
| 2 To complete the medium, aseptically and anaerobically distribute the medium into culture vessels containing steam-sterilized sulfur (see below, approximately 5.0 g/L) under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
Yeast extract
(10%) |
1.0 | ml | - | - | |
|
Glucose
(*, 1.0 M) |
10.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
2.5 | ml | - | - | |
Media with this solution
| J1287 | NATRONOANAEROARCHAEA MEDIUM WITH YEAST EXTRACT AND GLUCOSE |