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J1287. NATRONOANAEROARCHAEA MEDIUM WITH YEAST EXTRACT AND GLUCOSE

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Base salt medium 750.0 ml - -
2 Base soda medium 250.0 ml - -
3
(1 M)
1.0 ml 2.603 21.623
4 Trace element solution 1.0 ml - -
5 Selenite-tungstate solution
(*)
1.0 ml - -
6 Trace vitamins
(*)
5.0 ml - -
7
(10%)
1.0 ml - -
8
(*, 1.0 M)
10.0 ml - -
9
(5%)
2.5 ml - -
Instructions:
  1. Mix two media autoclaved separately. Aseptically add the following solutions (autoclaved or *filter-sterilized): #3-6
  2. To complete the medium, aseptically and anaerobically distribute the medium into culture vessels containing steam-sterilized sulfur (see below, approximately 5.0 g/L) under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized): #7-9
Instructions:
  1. Steam sulfur for 3 hr, or autoclave at 105C for 1 hr, on each of 3 successive days.
Solution occurs in 13 other media (first defined in medium J1079 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.00 g 2 7.194
3 0.10 g 0.1 -
4 0.03 g 0.03 -
5 0.30 g 0.3 4.852
6 0.20 g 0.2 -
7 0.01 g 0.01 -
8 0.02 g 0.02 -
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 3.6.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8
Solution occurs in 6 other media (first defined in medium J1207 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 240.0 g - -
2 5.0 g - -
3 2.5 g - -
4 0.5 g - -
Instructions:
  1. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with 0.5 M K2HPO4 and autoclave.
Solution occurs in 4 other media (first defined in medium J1207 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 190 g - -
2 30 g - -
3 16 g - -
4 1 g - -
Instructions:
  1. Add components to distilled water and bring volume to 1.0 L. Autoclave.

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 6.837 379.512
MgSO4 2.603 21.623
EDTA 0.005 0.017
FeSO4 x 7H2O 0.002 0.007
NaOH 3.9e-4 0.01
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