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JCM 197 . JCM MEDIUM No. 197

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J197 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.75 g 0.75 5.511
K2HPO4 0.75 g 0.75 4.306
MgCl2 x 6 H2O 0.36 g 0.36 1.771
NH4Cl 1.00 g 1 18.695
Yeast extract
(BD-Difco) 		0.10 g 0.1 -
Polypeptone
(Nihon Pharm. Co.) 		0.10 g 0.1 -
Sodium acetate 2.50 g 2.5 30.477
Trimethylamine-HCl 8.00 g 8 -
Trace mineral solution 9.00 ml - -
Trace vitamins 10.00 ml - -
NaHCO3 0.80 g 0.8 9.523
Na2S x 9 H2O 0.50 g 0.5 -
L-Cysteine HCl x H2O 0.50 g 0.5 -
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 981.00 ml - -
1 Mix ingredients except NaHCO3, Trace vitamins, Na2S x 9H2O and cysteine x HCl x H2O, and autoclave under a N2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as an 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O and cysteine x HCl x H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O and cysteine x HCl x H2O solutions, and adjust pH to 6.8.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace mineral solution #

1000
Solution occurs in 2 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 4.500 g 4.5 23.543
FeCl2 x 4 H2O 0.400 g 0.4 2.012
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.170 g 0.17 1.309
ZnCl2 0.100 g 0.1 0.734
CaCl2 x 2 H2O 0.020 g 0.02 0.136
H3BO3 0.019 g 0.019 0.307
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.030 g 0.03 0.231
Distilled water 1000.000 ml - -
1 Adjust pH to 7.0 with 1 N KOH.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trimethylamine-HCl 8 -
Sodium acetate 2.5 30.477
NH4Cl 1 18.695
NaHCO3 0.8 9.523
KH2PO4 0.75 5.511
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain