JCM 197 . JCM MEDIUM No. 197

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.75 g 0.75 5.511
K2HPO4 0.75 g 0.75 4.306
MgCl2 x 6 H2O 0.36 g 0.36 1.771
NH4Cl 1.00 g 1 18.695
Yeast extract
(BD-Difco)
0.10 g 0.1 -
Polypeptone
(Nihon Pharm. Co.)
0.10 g 0.1 -
Sodium acetate 2.50 g 2.5 30.477
Trimethylamine-HCl 8.00 g 8 -
Trace mineral solution 9.00 ml - -
Trace vitamins 10.00 ml - -
NaHCO3 0.80 g 0.8 9.523
Na2S x 9 H2O 0.50 g 0.5 -
L-Cysteine HCl x H2O 0.50 g 0.5 -
Resazurin 1.00 mg 1.0e-3 0.004
Distilled water 981.00 ml - -
1 Mix ingredients except NaHCO3, Trace vitamins, Na2S x 9H2O and cysteine x HCl x H2O, and autoclave under a N2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as an 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O and cysteine x HCl x H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O and cysteine x HCl x H2O solutions, and adjust pH to 6.8.
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Thiamine HCl 5.0 mg 0.005 -
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -
Solution occurs in 2 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 4.500 g 4.5 23.543
FeCl2 x 4 H2O 0.400 g 0.4 2.012
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.170 g 0.17 1.309
ZnCl2 0.100 g 0.1 0.734
CaCl2 x 2 H2O 0.020 g 0.02 0.136
H3BO3 0.019 g 0.019 0.307
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.030 g 0.03 0.231
Distilled water 1000.000 ml - -
1 Adjust pH to 7.0 with 1 N KOH.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trimethylamine-HCl 8 -
Sodium acetate 2.5 30.477
NH4Cl 1 18.695
NaHCO3 0.8 9.523
KH2PO4 0.75 5.511
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain