Main sol. J1312
Internal Solution-ID: S5472
Equipment needed: Anaerobic workbench
Filter
Autoclave
| 1 Mix ingredients, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add per liter the following solutions (filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.000 | g | 8.929 | 166.92 | |
| NaCl | 2.100 | g | 18.75 | 320.842 | |
| MgCl2 x 6 H2O | 0.825 | g | 7.366 | 36.232 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.893 | 6.073 | |
| KH2PO4 | 0.500 | g | 4.464 | 32.805 | |
| Trace element solution SL-10 | 1.000 | ml | - | - | |
| Selenite-tungstate solution | 1.000 | ml | - | - | |
|
Yeast extract
(BD-Difco) |
1.000 | g | 8.929 | - | |
|
Resazurin
(0.1%) |
0.500 | ml | - | - | |
| Trace vitamins | 10.000 | ml | - | - | |
|
NaHCO3
(8%) |
30.000 | ml | - | - | |
|
Taurine
(0.5 M) |
20.000 | ml | - | - | |
|
Sodium pyruvate
(1 M) |
20.000 | ml | - | - | |
|
Sodium L-lactate
(0.5 M) |
20.000 | ml | - | - | |
| Hemin solution | 10.000 | ml | - | - | |
| 2 Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine x HCl x H2O to the medium (3% solution, autoclaved and stored under a N2 atmosphere). | |||||
Media with this solution
| J1312 | MODIFIED MV MEDIUM |