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J1312. MODIFIED MV MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.000 g 8.929 166.917
2 2.100 g 18.75 320.828
3 0.825 g 7.366 36.233
4 0.100 g 0.893 6.073
5 0.500 g 4.464 32.805
6 Trace element solution SL-10 1.000 ml - -
7 Selenite-tungstate solution 1.000 ml - -
8
(BD-Difco)
1.000 g 8.929 -
9
(0.1%)
0.500 ml - -
10 Trace vitamins 10.000 ml - -
11
(8%)
30.000 ml 46.264 550.722
12
(0.5 M)
20.000 ml - -
13
(1 M)
20.000 ml - -
14
(0.5 M)
20.000 ml - -
15 Menadione solution 10.000 ml - -
Instructions:
  1. Mix ingredients, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add per liter the following solutions (filter-sterilized): #10-15
  2. Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine x HCl x H2O to the medium (3% solution, autoclaved and stored under a N2 atmosphere).
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium J862 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 -
3 4.0 mg 0.004 -
4 1000.0 ml 998.207 55408.8
Solution occurs in 20 other media (first defined in medium J433 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(25%, 7.7 M)
10.0 ml 3.17 86.943
2 1.5 g 1.5 -
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 -
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 -
7 2.0 mg 0.002 -
8 24.0 mg 0.024 -
9 36.0 mg 0.036 0.149
10 990.0 ml 988.225 54854.8
Instructions:
  1. Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.
Solution occurs in 4 other media (first defined in medium J469 )
Instructions:
  1. Dissolve 5 mg menadione first in 1 ml of ethanol. Then adjust volume to 100 ml.

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 106.862 5931.71
NaHCO3 46.264 550.722
NaCl 18.75 320.828
NH4Cl 8.929 166.917
Yeast extract 8.929 -
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