Main sol. 289
Internal Solution-ID: S556
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea water
(filtered) |
200.00 | ml | - | - | |
| Mineral salt solution | 150.00 | ml | - | - | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| Yeast extract | 0.60 | g | 0.6 | - | |
| K2HPO4 | 1.65 | g | 1.65 | 9.473 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Cellobiose | 5.00 | g | 5 | 14.607 | |
|
Cellulose
, Lens tissue or MN 301
optional |
5.00 | g | 5 | - | |
| Distilled water | 650.00 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine and cellobiose) and sparge medium with 100% N2 gas for 30 - 45 m in to make it anoxic. Add cysteine, adjust pH to 7.2 and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cellobiose from an anoxic stock solution sterilized by filtration. | |||||
| 2 Note: Some strains can be adapted to cellulose as substrate using 5.0 g/l cellulose (Lens tissue, OXOID; or cellulose powder MN 301, MACHEREY-NAGEL). If necessary adjust pH of completed medium to 7.2. | |||||
Media with this solution
| 289 | RUMINICLOSTRIDIUM PAPYROSOLVENS MEDIUM |