Main sol. 302
Internal Solution-ID: S5599
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , if necessary | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 To autoclaved medium add: | |||||
|
Horse serum
(sterile) |
100 | ml | - | - | |
| 2 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Media with this solution
| 302 | NUTRIENT BROTH WITH 10% HORSE SERUM |