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Solution A

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Internal Solution-ID: S5607

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.567 4.166
MgCl2 x 6 H2O 0.33 g 0.374 1.84
NaCl 0.40 g 0.454 7.76
NH4Cl 0.40 g 0.454 8.479
CaCl2 x 2 H2O 0.05 g 0.057 0.386
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Clarified rumen fluid , clarified 50.00 ml - -
Trypticase peptone
(BD BBL) 		1.00 g 1.134 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.7e-4 0.002
Distilled water 830.00 ml - -
397 FERULATE MEDIUM