Main sol. 673
Internal Solution-ID: S5645
Equipment needed: Hungate tubes
Autoclave
Gassing station
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
| KH2PO4 | 0.28 | g | 0.28 | 2.058 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
| FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.074 | |
| MnCl2 x 4 H2O | 1.80 | mg | 0.002 | 0.009 | |
| Na2B4O7 x 10 H2O | 4.50 | mg | 0.005 | - | |
| ZnSO4 x 7 H2O | 0.22 | mg | 2.2e-4 | 7.7e-4 | |
| CuCl2 x 2 H2O | 0.05 | mg | 5.0e-5 | 2.9e-4 | |
| Na2MoO4 x 2 H2O | 0.03 | mg | 3.0e-5 | 1.2e-4 | |
| VOSO4 x 2 H2O | 0.03 | mg | 3.0e-5 | 1.5e-4 | |
| CoSO4 x 7 H2O | 0.01 | mg | 1.0e-5 | 6.5e-5 | |
| NaCl | 25.00 | g | 25 | 427.789 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Yeast extract | 1.00 | g | 1 | - | |
|
Sulfur
(powdered) |
5.00 | g | 5 | 155.909 | |
| Distilled water | 1000.00 | ml | - | - | |
| Peptone | 5.00 | g | 5 | - | |
| 1 Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for a few minutes not autoclaved; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere. | |||||
| 2 Adjust final pH to 7.2. | |||||
Media with this solution
| 673 | THERMOCOCCUS PROFUNDUS MEDIUM |