Main sol. 1679
Internal Solution-ID: S5783
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl
(250 g/l stock solution) |
200.0 | g/l | 200 | 3422.31 | |
MgCl2 x 6 H2O
(200 g/l stock solution) |
20.0 | g/l | 20 | 98.375 | |
KCl
(50 g/l stock solution) |
20.0 | g/l | 20 | 268.276 | |
Mg(SO4) x 7 H2O
(350 g/l stock solution) |
20.0 | g/l | 20 | - | |
CaCl2 x 2 H2O
(50 g/l stock solution) |
20.0 | g/l | 20 | 136.041 | |
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
10.0 | ml/l | 0.006 | - | |
Na2-EDTA x 2 H2O
(0.1 g/l stock solution) |
10.0 | ml/l | 1.0e-3 | 0.003 | |
T-Trace Metal Mix | 1.0 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Adjust to 1000 mL with H2O dist. and autoclave. | |||||
2 After cooling, add the following filter sterilized components: | |||||
NaNO3
(300 g/l stock solution) |
2.5 | ml/l | 0.75 | 8.824 | |
K2HPO4 x 3 H2O
(8.0 g/l stock solution) |
2.5 | ml/l | 0.02 | 0.115 | |
Na2CO3
(20 g/l stock solution) |
1.0 | ml/l | 0.02 | 0.189 | |
Fe(NH4)citrate
(6.0 g/l stock solution) |
0.5 | ml/l | 0.003 | - | |
Vitamin B12
(0.02 g/l stock solution) |
1.0 | ml/l | 2.0e-5 | 1.5e-5 |
1679 | Cyanobacteria Medium 2xASN3 |