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Main sol. 1679

Internal Solution-ID: S5783

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl
(250 g/l stock solution)
200.0 g/l 200 3422.31
MgCl2 x 6 H2O
(200 g/l stock solution)
20.0 g/l 20 98.375
KCl
(50 g/l stock solution)
20.0 g/l 20 268.276
Mg(SO4) x 7 H2O
(350 g/l stock solution)
20.0 g/l 20 -
CaCl2 x 2 H2O
(50 g/l stock solution)
20.0 g/l 20 136.041
Na3-citrate x 2 H2O
(0.6 g/l stock solution)
10.0 ml/l 0.006 -
Na2-EDTA x 2 H2O
(0.1 g/l stock solution)
10.0 ml/l 1.0e-3 0.003
T-Trace Metal Mix 1.0 ml - -
Distilled water 1000.0 ml - -
1 Adjust to 1000 mL with H2O dist. and autoclave.
2 After cooling, add the following filter sterilized components:
NaNO3
(300 g/l stock solution)
2.5 ml/l 0.75 8.824
K2HPO4 x 3 H2O
(8.0 g/l stock solution)
2.5 ml/l 0.02 0.115
Na2CO3
(20 g/l stock solution)
1.0 ml/l 0.02 0.189
Fe(NH4)citrate
(6.0 g/l stock solution)
0.5 ml/l 0.003 -
Vitamin B12
(0.02 g/l stock solution)
1.0 ml/l 2.0e-5 1.5e-5
1679 Cyanobacteria Medium 2xASN3