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Main sol. 465 (modified)

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Internal Solution-ID: S5853

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.50 g 3.5 24.655
KH2PO4 1.00 g 1 7.348
MgCl2 x 6 H2O 0.10 g 0.1 0.492
Ca(NO3)2 x 4 H2O 0.05 g 0.05 0.212
Trace element solution SL-4 1.00 ml - -
Distilled water 1000.00 ml - -
1 pH 7.25
2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.
465d MINERAL MEDIUM WITH BENZYLCYANIDE AS NITROGEN SOURCE