Liquid medium (in 100 ml serum bottle)
Internal Solution-ID: S5859
Equipment needed: Serum bottle
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase soy broth
(BBL 11774) |
10.0 | ml | - | - | |
| 1 Prepare Trypticase Soy Broth (BBL 11774) under 80% N2 + 20% CO2 gas mixture in 100 ml serum bottles (10 ml per bottle). | |||||
| 2 After autoclaving and cooling add per bottle: | |||||
|
Glucose
(30% w/v; sterile) |
0.1 | ml | 0.03 | 0.167 | |
|
Bovine serum
(sterile; heat inactivated at 56°C for 45 min) |
0.7 | ml | - | - | |
|
NaHCO3
(10% w/v; sterile) |
0.6 | ml | 0.06 | 0.714 | |
| Air | 5.0 | ml | - | - | |
Media with this solution
| 840 | SERPULINA MURDOCHII MEDIUM |